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expression are associated with clinical phenotype and clinico-hematological response to hydroxyurea in essentialthrombocythemia

《医学前沿（英文）》 2022年 第16卷 第3期   页码 403-415 doi: 10.1007/s11684-021-0858-1

摘要： The morbidity and mortality of myeloproliferative neoplasms (MPNs) are primarily caused by arterial and venous complications, progression to myelofibrosis, and transformation to acute leukemia. However, identifying molecular-based biomarkers for risk stratification of patients with MPNs remains a challenge. We have previously shown that interferon regulatory factor-8 (IRF8) and IRF4 serve as tumor suppressors in myeloid cells. In this study, we evaluated the expression of IRF4 and IRF8 and the JAK2V617F mutant allele burden in patients with MPNs. Patients with decreased IRF4 expression were correlated with a more developed MPN phenotype in myelofibrosis (MF) and secondary AML (sAML) transformed from MPNs versus essential thrombocythemia (ET). Negative correlations between the JAK2V617F allele burden and the expression of IRF8 (P <0.05) and IRF4 (P<0.001) and between white blood cell (WBC) count and IRF4 expression (P <0.05) were found in ET patients. IRF8 expression was negatively correlated with the JAK2V617F allele burden (P <0.05) in polycythemia vera patients. Complete response (CR), partial response (PR), and no response (NR) were observed in 67.5%, 10%, and 22.5% of ET patients treated with hydroxyurea (HU), respectively, in 12 months. At 3 months, patients in the CR group showed high IRF4 and IRF8 expression compared with patients in the PR and NR groups. In the 12-month therapy period, low IRF4 and IRF8 expression were independently associated with the unfavorable response to HU and high WBC count. Our data indicate that the expression of IRF4 and IRF8 was associated with the MPN phenotype, which may serve as biomarkers for the response to HU in ET.

关键词： myeloproliferative neoplasms     IRF4     IRF8     hydroxyurea     essential thrombocythemia    

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Efficacy and safety of JAK inhibitor INC424 in patients with primary and post-polycythemia vera or post-essentialthrombocythemia myelofibrosis in the Chinese population

null

《医学前沿（英文）》 2016年 第10卷 第4期   页码 437-443 doi: 10.1007/s11684-016-0472-9

摘要：

A phase II study (A2202) was performed to evaluate the efficacy and safety of JAK inhibitor ruxolitinib in 63 Chinese MF patients. Ruxolitinib was given twice a day (bid) at a starting dose of 15 mg (n=25) or 20 mg (n=38) based on a baseline platelet count. About 94.7% of the patients achieved a reduction in spleen size, 27.0% of which exhibited significant reduction (≥35%) at week 24. Significant improvement in debilitating constitutional symptoms, as assessed by MFSAF v2.0, was observed in patients treated with ruxolitinib. Ruxolitinib treatment was generally well tolerated by Chinese patients. Although the treatment was associated with an increase in certain adverse events (AEs) that were established as identified risks (anemia and thrombocytopenia), these AEs were considered manageable in this clinical setting. Ruxolitinib provided substantial reductions in splenomegaly and improvements in symptoms, and was well-tolerated by Chinese patients with MF.

关键词： JAK     ruxolitinib     Chinese patients     myelofibrosis    

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Observation of a higher JAK2 V617F homozygous mutated clone in polycythemia vera compared to essentialthrombocythemia

LIU Hongxing, TONG Chunrong, CAI Peng, GU Jiangying, LIN Yuehui, TENG Wen, WANG He, ZHANG Ying, ZHU Ping

《医学前沿（英文）》 2008年 第2卷 第3期   页码 309-313 doi: 10.1007/s11684-008-0059-1

摘要： Single-tube bi-directional allele specific amplification (SB-ASA) and real-time quantitative polymerase chain reaction (RQ-PCR) assays were developed and performed for JAK2V617F detection on 40 polycythemia vera (PV) samples, 31 essential thrombocythemia (ET) samples, 40 acute leukemia samples, and 40 healthy control samples. Differences between detect limitations of the two assays and their influence on the mutation detection rate were analyzed. The results showed that in some samples, the JAK2V617F burden was as low as nearly 1%, and thus more JAK2V617F-positive samples were detected by RQ-PCR than by SB-ASA assay due to the former higher detect limitation. Mutation allele ratios in PV and ET samples and their relevance to biological characteristics were also analyzed. The results showed that the mutation allele ratio was 0.436 ± 0.261 in PV, higher than the 0.216 ± 0.207 in ET; percentage of certainly homozygous mutation carriers in PV was 40.54%, higher than the 10% in ET. However, statistical analysis showed no relevance between mutation allele burden and sex or age. Our result shows that the pathogenesis of PV and ET may be related to the mutation allele burden of JAK2V617F.

关键词： specific amplification     essential thrombocythemia     Single-tube bi-directional     JAK2V617F-positive     JAK2V617F detection    

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Overlap maximum matching ratio (OMMR): a new measure to evaluate overlaps of essential modules

Xiao-xia ZHANG,Qiang-hua XIAO,Bin LI,Sai HU,Hui-jun XIONG,Bi-hai ZHAO

《信息与电子工程前沿（英文）》 2015年 第16卷 第4期   页码 293-300 doi: 10.1631/FITEE.1400282

摘要： Protein complexes are the basic units of macro-molecular organizations and help us to understand the cell’s mechanism. The development of the yeast two-hybrid, tandem affinity purification, and mass spectrometry high-throughput proteomic techniques supplies a large amount of protein-protein interaction data, which make it possible to predict overlapping complexes through computational methods. Research shows that overlapping complexes can contribute to identifying essential proteins, which are necessary for the organism to survive and reproduce, and for life’s activities. Scholars pay more attention to the evaluation of protein complexes. However, few of them focus on predicted overlaps. In this paper, an evaluation criterion called overlap maximum matching ratio (OMMR) is proposed to analyze the similarity between the identified overlaps and the benchmark overlap modules. Comparison of essential proteins and gene ontology (GO) analysis are also used to assess the quality of overlaps. We perform a comprehensive comparison of serveral overlapping complexes prediction approaches, using three yeast protein-protein interaction (PPI) networks. We focus on the analysis of overlaps identified by these algorithms. Experimental results indicate the important of overlaps and reveal the relationship between overlaps and identification of essential proteins.

关键词： Protein-protein interaction network     Essential protein modules     Overlap     Overlap maximum matching ratio    

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森林火灾预报的新视角

赵宪文

《中国工程科学》 2000年 第2卷 第5期   页码 66-71

摘要：

从森林火灾成灾的三个基本因子（火源、环境和可燃物）入手，在宏观上对西南地区森林火灾预报方法进行了探讨。在预测林火发生方面，考虑到人为火是发生森林火灾的主要原因，有很大随机性，因而采用了马尔科夫随机过程理论。在分析成灾环境时，采用了与天、地、生相关分析的方法。特别在可燃物的贮量估测方面，用航天遥感数据，提出一套新的框算方法，为在区域的尺度上估测森林火灾给出了有效的、定量的方法，从而提高了可信度。

关键词： 森林火灾预报     航天遥感     火灾基本因子     新视角    

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Association study on GNB3 gene polymorphism with essential hypertension in Xinjiang Uygur group

JING Jianying, WANG Dan, WANG Xiaofeng, JIN Jianzhong, JIN Li, JIAO Yi, WEN Hao, LIN Renyong

《医学前沿（英文）》 2007年 第1卷 第2期   页码 230-233 doi: 10.1007/s11684-007-0045-z

摘要： The relationship between the tenth exon C825T of G-protein β3 subunit (GNB) genetic polymorphism and hypertension in the Uygur population of China was investigated. A nested case-control study ( = 738) was carried out. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to genotype GNB C825T polymorphism in 354 hypertensive (HT) and 384 normotensive (NT) Uygur subjects. The distributions of GNB C825T genotypes were CC (27.2%), TT (42.9%), and CT (29.9%) in the hypertensive subjects and CC (27.7%), TT (42.4%), CT (29.9%) in the normotensive subjects. There were no significant differences in the genotype distributions between the two groups ( = 0.0262 P = 0.99). The T allele was 51.4% in hypertensive subjects and 51.2% in normotensive subjects, which, between the two groups, was not a significant difference ( = 0.0016 P = 0.97). Further analysis shows that there is no association between C825T genotypes and age, body mass index (BMI), Glucose (GLU), Triglyceride (TG), Cholesterol (CHO), systolic blood pressure (SBP) and diastolic blood pressure (DBP). No evidence was found to suggest an association between GNB C825T polymorphism and hypertension in the Uygur population of China.

关键词： case-control     significant difference     reaction-restriction fragment     C825T polymorphism     evidence    

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Reactive oxygen species generation is essential for cisplatin-induced accelerated senescence in hepatocellular

null

《医学前沿（英文）》 2014年 第8卷 第2期   页码 227-235 doi: 10.1007/s11684-014-0327-1

摘要：

Accelerated senescence is important because this process is involved in tumor suppression and has been induced by many chemotherapeutic agents. The platinum-based chemotherapeutic agent cisplatin displays a wide range of antitumor activities. However, the molecular mechanism of cisplatin-induced accelerated senescence in hepatocellular carcinoma (HCC) remains unclear. In the present study, the growth inhibitory effect of cisplatin on HepG2 and SMMC-7721 cells was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Cellular senescence was then assessed by β-galactosidase assay. Senescence-related factors, including p53, p21, and p16, were evaluated by quantitative reverse transcription-polymerase chain reaction. Reactive oxygen species (ROS) was analyzed by flow cytometry. Our results revealed that cisplatin reduced the proliferation of HepG2 and SMMC-7721 cells in a dose- and time-dependent manner. Senescent phenotype observed in cisplatin-treated hepatoma cells was dependent on p53 and p21 activation but not on p16 activation. Furthermore, cisplatin-induced accelerated senescence depended on intracellular ROS generation. The ROS scavenger N-acetyl-L-cysteine also significantly suppressed the cisplatin-induced senescence of HepG2 and SMMC-7721 cells. In conclusion, our results revealed a functional link between intracellular ROS generation and cisplatin-induced accelerated senescence, and this link may be used as a potential target of HCC.

关键词： reactive oxygen species     senescence     cisplatin     hepatocellular carcinoma    

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网络安全研究——成功的数字化未来的关键

Jackie Craig

《工程（英文）》 2018年 第4卷 第1期   页码 9-10 doi: 10.1016/j.eng.2018.02.006

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Essential training steps to achieving competency in the basic intraoperative transesophageal echocardiography

null

《医学前沿（英文）》 2015年 第9卷 第1期   页码 123-128 doi: 10.1007/s11684-014-0366-7

摘要：

Guidelines for the intraoperative transesophageal echocardiography (TEE) examination have defined a detailed standard for medical professionals, particularly anesthesiologists, on how a TEE exam should proceed. Over the years, TEE has gained substantial popularity and emerged as a preferred monitoring modality to aid in perioperative management and decision making during hemodynamic instability situations or critical care settings. TEE training pathways and practice guidelines have been well established in western countries and many regions of the world. However, TEE training and practice information for anesthesiologists are lacking in China. As innovative technologies develop, other educational models have emerged to aid in obtaining competency in basic TEE exam. Hence, establishing a consensus on the ideal TEE training approach for anesthesiologists in China is urgently needed. Developing an effective curriculum that can be incorporated into an anesthesiology resident’s overall training is also necessary to provide knowledge and skills toward competency in basic TEE exam. With evolving medical system reforms and increasing demands for intraoperative hemodynamic monitoring to accommodate surgical innovations, anesthesiology professionals are increasingly obliged to perform intraoperative TEE exams in their current and future practices. To overcome obstacles and achieve significant progress in using the TEE modality to help in intraoperative management and surgical decision making, publishing basic TEE training guidelines for China’s anesthesiologists is an important endeavor.

关键词： transesophageal echocardiography     guidelines     training     competency    

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虚拟企业和谐机制研究

王硕,唐小我

《中国工程科学》 2004年 第6卷 第4期   页码 82-85

摘要：

文章运用和谐理论与方法，探讨虚拟企业运作与发展要素划分、现状和谐性分析，研究其和谐机制，据此可随时分析虚拟企业系统的和谐状态，以针对性改进其运行，最后给出案例研究。

关键词： 虚拟企业     和谐机制     协调     要素    

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is essential for the integrity of stereociliary rootlet in cochlear hair cells in mice

Yuqin Men, Xiujuan Li, Hailong Tu, Aizhen Zhang, Xiaolong Fu, Zhishuo Wang, Yecheng Jin, Congzhe Hou, Tingting Zhang, Sen Zhang, Yichen Zhou, Boqin Li, Jianfeng Li, Xiaoyang Sun, Haibo Wang, Jiangang Gao

《医学前沿（英文）》 2019年 第13卷 第6期   页码 690-704 doi: 10.1007/s11684-018-0638-8

摘要： encodes the taperin protein, which is concentrated in the tapered region of hair cell stereocilia in the inner ear. In humans, mutations cause autosomal recessive nonsyndromic deafness (DFNB79) by an unknown mechanism. To determine the role of in hearing, we generated -null mice by clustered regularly interspaced short palindromic repeat/Cas9 genome-editing technology from a CBA/CaJ background. We observed significant hearing loss and progressive degeneration of stereocilia in the outer hair cells of -null mice starting from postnatal day 30. Transmission electron microscopy images of stereociliary bundles in the mutant mice showed some stereociliary rootlets with curved shafts. The central cores of the stereociliary rootlets possessed hollow structures with surrounding loose peripheral dense rings. Radixin, a protein expressed at stereocilia tapering, was abnormally dispersed along the stereocilia shafts in null mice. The expression levels of radixin and -actin significantly decreased. We propose that is critical to the retention of the integrity of the stereociliary rootlet. Loss of in -null mice caused the disruption of the stereociliary rootlet, which resulted in damage to stereociliary bundles and hearing impairments. The generated -null mice are ideal models of human hereditary deafness DFNB79.

关键词： TPRN     stereocilia     stereociliary rootlet     actin filament     CRISPR/Cas9     hearing    

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流程制造业本质性分析

樊炯明,胡山鹰,陈定江,张群,李瑾,李光耀,宋晓旭,金涌,陈丙珍,殷瑞钰

《中国工程科学》 2017年 第19卷 第3期   页码 80-88 doi: 10.15302/J-SSCAE-2017.03.012

摘要：

为了能对流程制造业未来的发展方向有更加深刻的认识，本文从流程制造业中各行业的共性特点出发，将分析的视角从单一行业拓展到整个流程制造业，立足于热力学和熵等概念，对流程的本质及本质性特征进行了清晰深入的分析研究，构建了以“流”–“程”、“物质”–“能量”–“信息”、“规模”–“效率”三组重要因素为主的本质性分析框架，并对化工流程和磷化工流程案例进行了本质性分析。结果表明流程制造业发展的根本方向是要追求整体系统的熵增量趋向最小，而不是仅追求流程系统的熵减而忽视了外部环境系统中产生的熵增。要使整体系统的熵增趋向最小，不仅要关注流程系统的经济效益，同时也要提高资源和能源、环境效率。效率的提升本质上需要技术的改进、升级、创新来提供支撑，在流程系统中技术的改进升级可以小幅度提升流程系统的效率，而将颠覆性创新技术引入流程系统则可能使效率大幅度提升。

关键词： 流程制造业     熵     流程系统     本质     本质性特征    

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单萜类化合物芳樟醇、甲基丁香酚、草蒿脑和香茅醛对配体门控离子通道的作用模式 Article

Amy S. Li, Akimasa Iijima, Junhao Huang, Qing X. Li, Yongli Chen

《工程（英文）》 2020年 第6卷 第5期   页码 541-545 doi: 10.1016/j.eng.2019.07.027

摘要：

在传统的医学治疗中，精油被用作镇静剂、抗惊厥剂以及局部麻醉剂，还可以用作储藏食品、水果、蔬菜和谷物等的防腐剂，同时，它还能用作食品生产的生物农药。芳樟醇（LL）以及其他一些主要组分，如甲基丁香酚（ME）、草蒿脑（EG）和香茅醛是许多精油（如罗勒精油）中的活性化学成分。罗勒精油以及上述单萜类化合物在驱除害虫等方面有明显的功效。然而，这些化学成分的作用机制尚不明确。众所周知，γ-氨基丁酸A型受体（GABAAR）和烟碱型乙酰胆碱受体（nAChR）是目前市场上使用的合成杀虫剂的主要分子靶标。此外，GABAAR靶向治疗也已经有了数十年的临床应用实践，如巴比妥类药物和苯二氮类药物。在本研究中，我们研究了LL、ME、EG和香茅醛对GABAAR和nAChR的电生理作用，以进一步了解它们作为传统药物治疗剂和杀虫剂的多功能性。研究结果表明，LL对GABAAR和nAChR均有抑制作用，这或许可以解释LL的杀虫活性。LL是GABAAR的一种浓度依赖性、非竞争性抑制剂，因为在实验中我们发现，小鼠α1β3γ2L GABAAR的γ-氨基丁酸（GABA）半最大效应浓度（EC50）值不受LL影响：在LL不存在或存在5 mmol·L–1 LL的情况下，该半最大效应浓度值分别为(36.2 ± 7.9) μmol·L–1以及(36.1 ± 23.8) μmol·L–1。LL对GABAAR的半抑制浓度（IC50）约为3.2 mmol·L–1。考虑到在精油之中存在着多种单萜类化合物，所以LL可能与此前被表征为GABAAR激动剂和正变构调节物的ME以及其他单萜类化合物具有协同作用，这为LL的镇静、抗惊厥效用和杀虫活性提供了一种可能的解释。

关键词： 精油     γ-氨基丁酸A型受体     芳樟醇     单萜     烟碱型乙酰胆碱受体    

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基于情景构建的中国COVID-19相关基本临床医疗资源需求评估 Article

张婷, 王晴, 冷志伟, 杨媛, 杨津, 陈方媛, 贾萌萌, 张惺惺, 亓蔚然, 徐韵韶, 陈思雅, 戴佩希, 马礼兵, 冯录召, 杨维中

《工程（英文）》 2021年 第7卷 第7期   页码 948-957 doi: 10.1016/j.eng.2021.03.020

摘要：

新冠病毒肺炎（COVID-19）大流行是全球性的公共危机。COVID-19疫情暴发后，感染病例和重症患者骤增，导致亟需救治的患者数量增加、医疗资源供不应求，许多国家的医疗系统不堪重负，甚至崩溃。本研究基于不同情景下的COVID-19疫情暴发和防控，旨在评估中国COVID-19疫情相关的基本临床医疗资源需求。本研究使用易感者-潜隐者-传染者-住院/隔离者-移除者（包括康复和死亡）（SEIHR）传播动力学仓储模型来估计感染者和住院/隔离患者的数量，以及所需的基本医疗资源。研究发现，在实施严格的非药物干预（NPI）措施或大规模人群接种疫苗的情景下，中国能够迅速控制社区传播和局部地区暴发的聚集性疫情。然而，在实施较低强度的NPI措施和通过疫苗接种获得免疫的人口比例较低的情景下，需要使用平疫转换模式来储备医疗资源和提高服务能力，以确保疫情发生时医疗卫生系统的正常运行。不同时期COVID-19疫苗的接种和NPI措施的实施会减缓疫情的传播，进而影响临床救治需求。在构建的情景中，无症状感染者比例的增加不会减少对医疗资源的需求，但会增大疫情防控的难度。本研究为全球抗击COVID-19疫情期间防控策略的调整提供了依据，为未来应对COVID-19疫情大流行的公共卫生应急准备提供借鉴，也为基本医疗资源储备和配置提供指导。

关键词： COVID-19     SEIHR动力学模型     临床医疗资源需求     疫苗接种    

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标题 作者 时间 类型 操作

expression are associated with clinical phenotype and clinico-hematological response to hydroxyurea in essentialthrombocythemia

期刊论文

Efficacy and safety of JAK inhibitor INC424 in patients with primary and post-polycythemia vera or post-essentialthrombocythemia myelofibrosis in the Chinese population

null

期刊论文

Observation of a higher JAK2 V617F homozygous mutated clone in polycythemia vera compared to essentialthrombocythemia

LIU Hongxing, TONG Chunrong, CAI Peng, GU Jiangying, LIN Yuehui, TENG Wen, WANG He, ZHANG Ying, ZHU Ping

期刊论文

Overlap maximum matching ratio (OMMR): a new measure to evaluate overlaps of essential modules

Xiao-xia ZHANG,Qiang-hua XIAO,Bin LI,Sai HU,Hui-jun XIONG,Bi-hai ZHAO

期刊论文

森林火灾预报的新视角

赵宪文

期刊论文

Association study on GNB3 gene polymorphism with essential hypertension in Xinjiang Uygur group

JING Jianying, WANG Dan, WANG Xiaofeng, JIN Jianzhong, JIN Li, JIAO Yi, WEN Hao, LIN Renyong

期刊论文

Reactive oxygen species generation is essential for cisplatin-induced accelerated senescence in hepatocellular

null

期刊论文

网络安全研究——成功的数字化未来的关键

Jackie Craig

期刊论文

Essential training steps to achieving competency in the basic intraoperative transesophageal echocardiography

null

期刊论文

虚拟企业和谐机制研究

王硕,唐小我

期刊论文

is essential for the integrity of stereociliary rootlet in cochlear hair cells in mice

Yuqin Men, Xiujuan Li, Hailong Tu, Aizhen Zhang, Xiaolong Fu, Zhishuo Wang, Yecheng Jin, Congzhe Hou, Tingting Zhang, Sen Zhang, Yichen Zhou, Boqin Li, Jianfeng Li, Xiaoyang Sun, Haibo Wang, Jiangang Gao

期刊论文

流程制造业本质性分析

樊炯明,胡山鹰,陈定江,张群,李瑾,李光耀,宋晓旭,金涌,陈丙珍,殷瑞钰

期刊论文

单萜类化合物芳樟醇、甲基丁香酚、草蒿脑和香茅醛对配体门控离子通道的作用模式

Amy S. Li, Akimasa Iijima, Junhao Huang, Qing X. Li, Yongli Chen

期刊论文

基于情景构建的中国COVID-19相关基本临床医疗资源需求评估

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